N-substituted amino-n-nitroso-aminoacetonitriles for treating hypertension

ABSTRACT

Certain known N-substituted amino-N-nitroso-aminoacetonitriles, e.g., N-morpholino-N-nitroso-aminoacetonitrile, have been found to be useful as hypotensive/anti-hypertensive agents.

United States Patent [191 Anderson Dec. 3, 1974 N-SUBSTITUTED [58] Field of Search 424/250 AMINO-N-NITROSO- AMINOACETONITRILES FOR TREATING [56] References Cited HYPERTENSION Paul L. Anderson, Dover, NJ.

Assignee: Sandoz-Wander, lnc., Hanover, NJ.

Filed: Aug. 22, 1973 Appl. No.: 390,531

Related US. Application Data Division of Ser. Nos. 326,405. Feb. 4, 1971, Pat. No. 3,780,180. which is a continuation-in-part of Ser. No. 41,056. May 27, 1970, abandoned.

Inventor:

US. Cl 424/250, 424/244, 424/248, 424/258, 424/267, 424/304 Int. Cl A6lk 27/00 OTHER PUBLICATIONS Derwent No. 30749, abstracting Netherlands 67/0945 (Feb. 12, 1968).

Primary Examiner-Stanley J. Friedman Attorney, Agent, or FirmGerald D. Sharkin; Robert S. Honor [57] ABSTRACT Certain known N-substituted amino-Nnitrosoaminoacetonitriles, e.g., N-morpholino-N-nitrosoaminoacetonitrile, have been found to be useful as hypotensive/anti-hypertensive agents.

2 Claims, No Drawings The active agents with which this invention is concerned may be represented by the following structural formula where R represents hydrogen, alkyl or where m is O, 1 or 2; and

R and R each, independently, represent lower alkyl, i.e., alkyl having 1 to 4 carbon atoms, e.g., methyl, ethyl, n-propyl and the like; lower alkenyl, i.e., alkenyl having 3 to carbon atoms, e.g., allyl, and the like; lower alkynyl, i.e., alkynyl having 3 to 5 carbon atoms; e.g., propargyl and the like; w-hydroxyloweralkyl, i.e., w-hydroxyloweralkyl having 2 to 4 carbon atoms, e.g., B-hydroxyethyl and the like; cycloalkyl having 3 to 8 carbon atoms, e.g., cyclohexyl and the like;

to 36; cyano trifluoromethyl; lower alkyl as defined above; lower alkoxy i.e., alkoxy having 1 to 4 carbon atoms, e.g., methoxy, ethoxy, and the like; alkanoyloxy having 2 to 4 carbon atoms, e.g., acetoxy and the like; alkanoyl having 2 to 4 carbon atoms, e.g., acetyl and the like; w-hydroxy-lower alkyl, i.e., w-hydroxy-lower alkyl having 2 to 4 carbon atoms, e.g., B-hydroxyethyl and the like; vdilower alkyl amino, i.e., di-lower alkyl amino wherein the lower alkyl is as defined above, e.g., dimethylamino, diethylamino and the like; or

R and R together on adjacent atoms may be -CH- OCH or -OCH O; or

R and R together with N represent in (Wi (In) (0) n gm 11 where n is 4, 5 or 6; p is 0, 1 or 2; X represents -O or where R is hydrogen; lower alkyl as defined above; alkanoyl having 2 to 4 carbon atoms; w-hydroxy lower alkyl as defined above; alkoxyalkyl having 2 to 4 carbon atoms, e.g., methoxyethyl and the like; lower alkyl having 1 to 4 carbon atoms substituted with 1 to 4 halogen atoms having an atomic weight between 19 to 36, e.g., trifluoromethyl and the like where m is as defined above; and

R represents hydroxy; halo as defined above; lower alkyl, as defined above; lower alkoxy, as defined above, alkanoyl having 2 to 4 carbon atoms; alkanoyloxy having 2 to 4 carbon atoms; w-hydroxy lower alkyl, as defined above, alkoxyalkyl having 2 to 4 carbon atoms, e.g., methoxyethyl and the like; lower alkyl having 1 to 4 carbon atoms substituted with l to 4 halogens having an atomic weight between about 19 to 36, e.g., trifluoromethyl, afi-dichloroethyl and the like; or

where m is as defined above, provided that when R and R are both trifluoromethyl or when more than one of R is trifluoromethyl on a phenyl ring, they are on other than adjacent carbon atoms.

The compounds of formula (I) are known and may be prepared according to methods disclosed in the literature from known materials. The present invention contemplates only the novel use of such compounds, particularly as hypotensive/anti-hypertensive agents. The preferred compound for this use is N-morpholino- N-nitroso-aminoacetonitrile.

Other N-disubstituted amino-N-nitrosoaminoacetonitriles which can be used in the pharmaceutical compositions of this invention include N-diallylamino-N-nitroso-aminoacetonitrile N-dimethylamino-N-nitroso-aminoacetonitrile N-diallylamino-N-nitroso-a-phenethylaminoacetonitrile N-diallylamino-N-nitroso-a-methylaminoacetonitrile N-methylpropargylamino-N-nitrosoaminoacetonitrile N-(N-allyl-B-hydroxyethylamino)-N-nitrosoaminoacetonitrile N-methylcyclohexylamino-N-nitrosoaminoacetonitrile N-methylphenylamino-N-nitroso-aminoacetonitrile N-methylphenylethylamino-N-nitrosoaminoac etonitrile N-dibenzylamino-N -nitrso-aminoacetonitri le N-( N-methyl-p-hydroxyphenylamino )-N-nitrosoaminoacetonitrile N-( N-propyl-p-chlorophenylamino )-N-ni trosoaminoacetonitrile N-( N-methyl-p-cyanophenylamino )-N-nitroso-aphenyl-aminoacetonitrile N-( N-ethyl-o-trifl uoromethylphenylamino -N- nitroso-aminoacetonitrile N-( N-methyl-o-methoxyphenylamino )-N-nitrosoaminoacetonitrile N-( N-methyl-o-toluidino )-N-nitrosoaminoacetonitrile N-( N-methyl-p-acetoxyphenylamino )-N-nitrosoaminoacetonitrile N-( N-methyl-p-acetylphenylamino )-N-nitrosoaminoacetonitrile N-( N-methyl-o- B-hydroxyethyl phenylamino )-N nitroso-aminoacetonitrile N-( N-methyl-p-dimethylaminophenylaminoJ-N- 4 nitroso-aminoacetonitrile N-( N-rnethyl-3 ,4-dimethyleneoxyphenylamino )-l\lnitroso-aminoacetonitrile and N-( N-methyl-3 ,4-methylenedioxybenzylamino)-N- nitroso-aminoacetonitrile The N-heterocyclic substituted-N-nitrosoaminoacetonitriles contemplated as active agents in the compositions of this invention include N-piperidino-N-nitroso-aminoacetonitrile N-piperidino-N-nitroso-ck-methyl-amlnoacetonitrile N-hexamethyleneimino-N-nitroso-aminoacetonitrile N-(4-hydroxypiperidino)-N-nitrosoaminoacetonitrile N-( 2,4-dic hloropiperidino )-N-nitrosoaminoacetonitrile N-(4-methylpiperidino)-N-nitroso-arninoacetonitrile N-( 4-methoxypiperidino )-'N-nitr0soaminoactonitrile N-( 4-actylpiperidino )-N-nitrosoaminoacetonitrile N-( 4-acetoxypiperidino -Nnitrosoaminoacetonitrile N-( 4-[B-hydroxyethyl ]-piperidino)-N-nitrosoaminoacetonitrile N-( 4-methoxyethylpiperidino )-N-nitrosoaminoac etonitrile N-( 4-trifluoromethylpiperidino )-N-nitrosoaminoacetonitrile N-( 2-benzylpiperidino )-N-nitroso-aminoacetonitrile N-( Z-phenylpiperidino )-N-nitroso-aminoacetonitrile N-isoquinolino-N-nitroso-aminoacetonitrile N-[ l-hydroxyisoquinolino1-N -nitrosoaminoacetonitrile N-[ l-chloroisoquinolino]-N-nitrosoaminoacetonitrile N-[ l-methylis0quinolino]-N-nitrosoaminoacetonitrile N-[ l-meth0xyisoquinolino]-N-nitrosoaminoacetonitrile N-{ l-acetylisoquinolino]-N-nitr0soaminoacetonitrile N-[ l-acetoxyisoquinolin0]-N-nitrosoaminoacetontrile N-[ l-B-hydroxyethylisoquinolino]-N-nitrosoaminoacetonitrile N-[ l-methoxyethylisoquinolino -N-nit'rosoaminoacetonitrile N- [4-trifl uoromethyl isoquinolino -N-nitrosoaminoacetonitrile N-[ l-benzylisoquinolino -N-nitrosoaminoacetonitrile N-( 2-hydroxyrnorpholino )-N-nitrosoaminoacetonitrile N-( 2-chloromorpholino )-N-nitrosoaminoacetonitrile N-( Z-methylmorpholino )-N-nitrosoaminoacetonitri le N-( Z-methoxymorpholino )-N-nitrosoaminoacetonitirle N-( Z-acetylmorpholino )-N-nitro-aminoace tonitri le N-( Z-acetoxymorpholino )-N-nitrosoaminoacetonitrile N-( 3-B-hyd roxyeth ylmorpholino )-N-nitrosoaminoacetonitrile N-( 3 -methoxyethylmorpholino )-N-nitrosoaminoacetonitrile N-( 3-trifluoromethylmorpholino )-N-nitrosoaminoacetonitrile N-( 3-benzylmorpholino )-N-nitrosoaminoacetonitrile tN-piperazino-N-nitroso-aminoacetonitrile N- 2-hyd roxypiperazino -N-nitrosoaminoacetonitrile N- 2-chloro-4-methylpipe razi no -N-nitrosoaminoacetonitrile N-[ 2-rnethyl-4-acetylpiperazino -N-nitros0- aminoacetonitrile N- [2-methoxypiperazino 1 -N-nitrosoaminoacetonitrile N 2-acetylpiperazino 1 -N-nitroso-aminoacetonitrile N-[ 2-acetoxypiperazino -N-nitrosoaminoacetonitrile N- 2-Bhydroxyethylpiperazino -N-nitrosoaminoacetonitrile N-[2-methoxyethylpiperazino -N-nitrosoaminoacetonitrile N-[ 3-trifluoromethylpiperazino -N-nitrosoaminoacetonitrile N-[ 3 -benzylpiperazino -N-nitroso-aminoacetonitrile N-[4-3-hydroxyethylpiperazino]-N-nitrosoaminoacetonitrile N-[ 4-methoxyethylpiperazino ]-N -nitrosoaminoacetonitrile N-[4-trifluoromethylpiperazinol-N-nitrosoaminoacetonitrile N-{4-benzylpiperazino1-N-nitroso-aminoacetonitrile N-[4-B-hydroxyethylpiperazino]-N-nitroso-amethyl-aminoacetonitrile.

As previously indicated, the compounds of formula (I) are useful because they possess pharmacological acitivity in animals. In particular, they are useful as hypotensive/anti-hypenensive agents asindicated by their activity in renal hypertensive rat given 30 mg/kg of active compound using the technique of A. Grollman (Proc. Soc. Exptl. Biol. and Med. 571102, 1944) and indirectly measuring the blood pressure from the caudal artery in the tail using a pneumatic pulse transducer. For such usage, compound (I) may be administered orally or parenterally as such or admixed with conventional pharmaceutical carriers. They may be administered orally in such forms as tablets, dispersible powders, granules, capsules, syrups and elixirs, and parenterally as solutions, suspensions, dispersions, emulsions, and the like, e.g., a sterile injectable aqueous suspension. The compositions for oral use may contain one or more conventional adjuvants, such as sweetening agents, flavoring agents, coloring agents and preserving agents, in order to provide an elegant and palatable preparation. Tablets may containthe active ingredient in admixture with conventional pharmaceutically acceptable excipients, e.g., inert diluents, such as calcium carbonate, sodium carbonate, lactose and talc, granulating and disintegrating agents, e.g.,

. starch and alginic acid, binding agents, e.g., starch, gelatin and acacia, and lubricating agents, e.g., magnesium stearate, stearic acid and talc. The tablets may be uncoated or coated by known techniques to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. Similarly, suspensions, syrups and elixirs may contain the active ingredient in admixture with any of the conventional excipients utilized for the preparation of such compositions, e.g., suspending agents (methylcellulose, tragacanth and sodium alginate), wetting agents (lecithin, polyoxyethylene stearate and polyoxyethylene sorbitan monooleate) and preservatives (ethyl-p-hydroxybenzoate). Capsules may contain the active ingredient alone or admixed with an inert solid diluent, e.g., calcium carbonate, calcium phosphate and kaolin. The injectable compositions are formulated as known in the art and may contain appropriate dispersing or wetting agents and suspending agents identical or similar to those mentioned above. The pharmaceutical preparations may contain up to about 90 percent of the active ingredient in combination with the carrier or adjuvant.

The amt-hypertensive effective dosage of active ingredient employed for the treatment of hypertension may vary depending on the particular compound employed and the severity of the condition being treated. However, in general, satisfactory results are obtained when the compounds (I) are administered at a daily dosage of from about 0.1 milligram to about 50 milligrams per kilogram of animal body weight, preferably given in divided doses 2 to 4 times a day, or in sustained release form. For most large mammals in need of said treatment, the total daily dosage is from about 7 to about 200 milligrams. Dosage forms suitable for internal use comprise from about l.75 to about 100 milligrams of the active compound in intimate admixture with a solid or liquid pharmaceutically acceptable carrier or diluent.

The preferred pharmeceutical compositions from the standpoint of preparation and ease of administration are solid compositions, particularly hard-filled capsules and tablets containing about 25 to 100 milligrams of active ingredient.

EXAMPLE 1 ventional tabletting techniques. Such tablets are useful in treating hypertension at a dose of one tablet 2 to 4 times a day.

ingredient Weight (mg) N-morpholino-N-nitroso-aminoacetonitrile 50 Tragacanth l0 Lactose 197.5 Corn Starch 25 Talcum l5 Magnesium Stearate 2.5

EXAMPLE 2 Dry Filled Capsules Capsules suitable for oral administration which contain the following ingredients are prepared in a conventional manner. Such capsules are useful in treating hypertension at a dose of one-capsule 2 to 4 times a day.

Ingredient Weight (mg) N-morpholino-N-nitroso-arninoacetonitrile 50 inert solid diluent (starch, lactose, kaolin) 250 EXAMPLE 3 Sterile Solution for Injection The following ingredients are dissolved in water for injection. The resulting solution is filtered through an appropriate medium to render a clear solution. The solution is then autoclaved to render it sterile.

Ingredient Weight N-morpholino-N-nitroso-aminoacetonitrile 10 Sodium alginate 0.5 Buffer system As desired Lecithin 0.5 Sodium chloride As desired Water for injection To desired volume EXAMPLE 4 The following formulations for syrups or elixirs containing an effective amount of active compound may be formulated using conventional methods.

Percent by Weight EXAMPLES and 6 Tablets and Capsules Suitable For Oral Administration Tablets and capsules containing the ingredients indicated belowmay be prepared by conventional techniques and are useful in treating hypertension at a dose of one tablet or capsule 2 to 4 times a day.

Ingredient Weight (mg) tablet capsule N-(Z-chloromorpholino)-N-nitrosoaminoacetonitrile tragacanth lactose corn starch talcum magnesium stearate 2.

Total 300 mg Similarly, tablets and capsules are prepared using N- (2-hydroxymorpholino)-N-nitroso-aminoacetonitrile; N-(2-inethylmorpholino)-N-nitroso-aminoacetonitrile; N-(Z-methoxymorpholino)-N-nitrosoaminoacetonitrile; N-(Z-acetylmorpholino)-N-nitrosoaminoacetonitrile or N-(2-acetoxymorpholino)-N- nitroso-aminoacetonitrile in place of the N-(2- chloromorpholino)-N-nitroso-aminoacetonitrile above and are used as above in treating hypertension.

EXAMPLE7 and s Sterile Suspension for Injection and Oral Liquid Suspension v I The following pharmaceutical compositions are formulated with the indicated amount of active agent using conventional techniques. The injectable suspension and the oral liquid suspension represent formulations useful as unit doses and may be administered in the treatment of hypertension. The injectable suspension is suitable for administration once or twice a day whereas the oral liquid suspension is suitably administered 2 to 4 times per day for this purpose.

tritluoromethylmorpholino)-N-nitroso aminoacetonitrile or N-(3-benzylmorpholino)-N- nitroso-aminoacetonitrile in place of the N-(2- hydroxymorpholino)-N-nitroso-aminoacetonitrile above and are used as above in treating hypertension.

7 EXAMPLES 9 and 10 I Tablets and Capsules Suitable For Oral Administration Tablets and capsules containing the ingredients indicated below may be prepared by conventional techniques and are useful in treating hypertension at a dose of one tablet or capsule 2 to 4 times a day.

Ingredient Weight (mg) tablet capsule N diallylamino N-nitroso-amino 50 acetonitrile tragacanth l0 lactose 197.5 250 corn starch 25 talcum l5 magnesium stearate 2.5

Total 300 mg. 300 mg.

Weight (mg) sterile ingredients injectable suspension oral liquid suspension N-(Z-hydroxymorpholino)-N-nitrosoaminoacetonitrile sodium carboxy methyl cellulose U.S.P. methyl cellulose olyvinylpyrrolidone ecithin I benzyl alcohol magnesium aluminum silicate flavor color methyl paraben, U.S.P. propyl paraben. U.S.P. polysorbate 80 (e.g. Tween 80), U.S.P. sorbitol solution, 70%. U.S.P. buffer agent to adjust pH for desired stability water III I l l l w ys o .s. for in ection. q.s. to lml.

lll

q.s. to 5 ml.

lnjectable suspension and oral liquid suspensions are similarly prepared using N-(2-(chloromorpholino)-N- nitroso-aminoacetonitrile; N-(2-methylmorpholino)- N-nitroso-aminoacetonitrile; N-( 3-B- hydroxymethylmorpholino)-N-nitrosoaminoacetonitrile; N-(3-methoxymethylmorpholino)- N -nitroso-aminoacetonitrile; N-( 3- EXAMPLES 1 l and 12 Tablets and Capsules Suitable For Oral Administration Tablets and capsules containing the ingredients indicated below may be prepared by conventional techniques and are useful in treating hypertension at a dose of 'one tablet or capsule 2 to 4 times a day.

N-dibenzylamino-N-nitroso-aminacetonitrile;

Ingredient Weight (mg) tablet capsule N-methylphenylamino-N-nitroso-amino- 50 50 acetontrile tragacanth 10 lactose 197.5 250 corn starch talcum l5 magnesium stearate 2.5

Total 300 mg. 300 mg.

25 ing hypertension.

. EXAMPLES 13 and 14 Sterile Suspension for lnjection and Oral Liquid Suspension The following pharmaceutical compositions are formulated with the indicated amount of active agent using conventional techniques. The injectable suspension and the oral liquid suspension represent formulations useful as unit doses and may be administered in the treatment of hypertension. The injectable suspension is suitable for administration once a day whereas the oral liquid suspension is suitably administered 2 to 4 times per day for this purpose.

methyl-3,4-dimethyleneoxyphenylamino)-N-nitrosoaminoacetonitrile; or N-(N-methyl-3,4- methylenedioxybenzylamino)-N-nitrosoaminoacetontrile in place of the N-(N-methyl-otoluidino) N-nitrosoaminoacetonitrile above and are used as above in treating hypertension.

EXAMPLES 15 and 16 Tablets and Capsules Suitable For Oral Administration Tablets and capsules containing the ingredients indicated below may be prepared by conventional tech niques and are useful in treating hypertension at a dose of one tablet 0r capsule 2 to 4 times a day.

Ingredient Weight (mg) tablet capsule N-piperidino-N-nitroso-aminoaceton- 50 itrile tragacanth 10 lactose 197.5 250 corn starch 25 talcum l5 magnesium stearate 2.5

Total 300 mg. 300 mg.

Similarly, tablets and capsules are prepared using N- piperidino-N-nitroso-a-methyl-aminoacetonitrile; N- hexyleneimino-N-nitroso-aminoacetonitri1e; N-(4- hydroxypipe ridino )-N-nitroso-aminoacetonitrile; N-

(2,4-dichloropiperidino)-N-nitroso-aminoacetonitrile; N-(4-methylpiperidino)-N-nitroso-aminoacetonitri1e; N-(4-meth0xypiperidino)-N-nitroso-aminoacetonitrile; N-(4-acetylpiperidino)-N-nitroso-aminoacetonitrile; or N-(4-acetoxypiperidino)-N-nitroso-aminoacetonitrile, in place of the N-piperazino-N-nitrosoaminoacetonitrile above and are used as above in treat- Ingredients sterile injectable suspension Weight (mg) oral llqUlCl suspension N-(N-methyl-o-toluidino) N-nitrosoaminoacetonitrile sodium carboxy methyl cellulose U.S.P. methyl cellulose polyvinylpyrrolidone lecithin benzyl alcohol magnesium aluminum silicate color methyl paraben, U.S.P.

propyl paraben, U.S.P.

polysorbate 80 (e.g. Tween 80). U.S.P. sorbitol solution. 70%, U.S.P.

buffer agent to adjust pH for desired stability water LII | l I uma a O q.s. for injection, q.s. to 1 ml.

q.s. to 5 ml.

Sterile injectable suspensions and oral liquid suspensions are similarly prepared using N-(N-methyl-lacetoxyphenylamino)-N-nitroso-aminoacetonitrile; N- N-methyl-p-actylphenylamino)-N-nitrosoaminoacetonitrile; N-(n-methyl-o-[B- hydroxyethyl lphenylamino-N-nitrosoaminoacetonitrile; N-(N-methyl-p-dimethylaminophenylamino)-N-nitroso-aminoacetonitrile; N-(N- ing hypertension.

EXAMPLES 17 and 18 Sterile Suspension for Injection and Oral Liquid Suspension i The following pharmaceutical compositions are formulated with the indicated amount of active agent using conventional techniques. The injectable suspension and the oral liquid suspension represent formulations useful as unit doses and may beadministered in the treatment of hypertension. The injectable suspension is suitable for administration once a day whereas the treatment of hypertension. The injectable suspen-' the oral liquid suspension is suitably administered 2 to 5 sion is suitable for administration once a day whereas 4 times per day forthis purpose. the oral liquid suspension is suitably administered 2 to Weight lmg) sterile oral Ingredients injectable suspension liquid suspension aminoacetonitrile nitroso aminoacetonitnle 50 50 sodium carboxy methyl cellulose U.S.P. 1.25 12.5 methyl cellulose 0.4 polyvinylpyrrolidone 5 lecithin 3 benzyl alcohol 001 magnesium aluminum silicate 47.5 flavor q.s. color q.s. methyl paraben, U.S.P. 4.5 propyl paraben, U.S.P. 1.0 polysorbate 80 (cg. Tween 80), U.S.P. 5 sorbitol solution, 70%, U.S.P. 2,500 buffer agent to adjust pH for desired stability q.s. q.s. water for injection,

q.s. to 1 ml. q.s. to 5 ml.

Sterile injectable suspensions and oral liquid suspen- 4 times per day for this purpose.

mmg) sterile oral Ingredients injectable suspension liquid suspension N-isoquinolino-N-nitroso-aminoacetonitrile 50 50 sodium carboxy methy cellulose U.S.P. 1.25 12.5 ,methylcellulose 0.4 polyvinylpyrrolidone 5 lecithin 3 benzyl alcohol 0.01 magnesium aluminum silicate 47.5 flavor q.sr color q.s. methyl paraben, U.S.P. 4.5 propyl paraben, U.S.P. L polysorbate 80 (eg. Tween 80), U.S.P. S sorbitol solution, 70%; U.S.P. 2,500 buffer agent to adjust pH for desired stability q.s. q.s. water for injection.

' q.s. to 1 ml. q.s. to ml.

sions similarly are prepared using N-(4-[B- hydroxyethyl]piperidino)-N-nitroso-a-methyl aminoacetonitrile; N-(4-methoxyethylpiperidino)-N- nitroso-aminoacetonitrile; N-(4- trifluoromethylpiperidino)-N-nitrosoamirioacetonitrile; N-( 2-benzylpiperidino )-N-nitrosoor N-(2-phenylpiperidino)-N- nitroso-aminoacetonitrile in place of the N-(4-[B- hydroxyethyl]piperazino)-N-nitroso-aminoacetonitrile above and are used as above in treating hypertension.

EXAMPLES l9 and Sterile Suspension fo r Injection and Oral Liquid S uspension Sterile injectable suspensions'and oral liquid suspensions similarly are prepared using N-(lhydroxyisoquinolino)-N-nitroso-aminoacetonitrile; N- l-chloroisoquinolino )-N-nitroso-aminoacetonitrile; N-( l-methylisoquinolino )-N-nitrosoaminoacetonitrile; N-( l-methoxyisoquinolino )-N- nitroso-aminoacetonitrile or N-( l-acetylisoquinolino)- N-nitroso-aminoacetonitrile in place of N-isoquinolino- N-nitroso-aminoacetonitrile above and are used as above in treating hypertension.

EXAMPLES 21 and 22 Tablets and Capsules Suitable For Oral Administration Tablets and capsules containing the ingredients indicated below may be prepared by conventional techniques and are useful in treating hypertension at a dose of one tablet or capsule 2 to 4 times a clay.

Ingredient Weight gmg) tablet capsule N-( lBhydroxyethylisoquinolino)-N- nitrosoaminoacetonitrile 50 5O tragacanth l lactose 197.5 250 corn starch 25 talcum l magnesium stearate 2.5

Total 300 mg. 300 mg.

Similarly tablets and capsules are prepared using N- EXAMPLES 25 and 26 Tablets and Capsules Suitable For Oral Administration Ingredient Weight (mg) l -acetoxy-isoquinolino )-N-nitroso-aminoacetonitrile; labia capsule N-( l-methoxyethylisoquinolino-N-nitroso- N B h a h I v N y roxyet ypiperazino aminoacetonitrile, N 4 trifluoromethylisoquinolino) 15 nitmsoaminoacemnimle 50 50 N-nitroso-aminoacetonitrile; or N-( 1- tragacanth 10 benzylisoquinolino)-N-nitroso-aminoacetonitrile as the lactose 250 corn starch 25 active ingredient in place of the N-( l-B- talcum 7 l5 hydroxyethylisoquinolino)-N-nitrosomagnesium Swarm T i 25 aminoacetonitrile above and are used as above in treat- 300 300 ing hypertension.

EXAMPLES 23 and 24 Similarly, tablets and capsules are prepared using N- stenle Suspension for lnlectlon and Oral Llquld (3-benzylpiperazino)-N-nitroso-aminoacetonitrile; N- pension 25 (4-methoxyethylpip'erazino)-N-nitr0so- The following pharmaceutlcal composition? are aminoacetonitrile; N-(4-trifluoromethylpiperzino)-N- mulamd W1th the mdlcafied amoun't P acme agent nitroso-aminoacetonitrile; N-(4 benzylpiperazino)-N- using conventional techniques. The in ectable suspeni i i il or 4 SlOll and th oral SUSPCl'lSlOl'l represent formulahydroxyethylpipe azino)..N-nitr so-a methyl tions useful as unit doses and may be administered in i it il N-(4- 3-h d h i i the treatment of hypertension. The lIljCCtflbi suspenit sQ-a-phenyl-aminoacetonitrile in' place of the N- sion is suitable for administration once a day whereas (4-5-h d th l i i )-N- i the oral liquid suspension is suitable administered 2 to i t it il above d are d as above i treat- 4 times per day for this purpose. ing hypertension.

Weight 1mg) sterile oral Ingredients injectable suspension liquid suspension N-piperazino-N-nitroso-aminoacetonitrile 50 sodium carboxy methyl cellulose U.S.P. 1.25 l2.5 methyl cellulose 0.4

olyvinylpyrrolidone 5 ecithin 3 benzyl alcohol 0.0l magnesium aluminum silicate 47..5 flavor q.s. color q.s. methyl paraben, U.S.P. 4.5 propyl paraben. U.S.P. l.O polysorbate 80 (e.g. Tween 80). U.S.P. 5 sorbitol solution. 70%. U.S.l?. 2,500 buffer agent to adjust pH for desired stability .5. q.s. water for injection.

-q.s. to l ml. q.s. to 5 ml.

Sterile injectable suspensions and oral liquid suspen- What is claimed is:

sions are similarly prepared using 'N-(2- l. A methodfor treating hypertension, which comhydroxypiperazino)-N-nitroso-aminoacetonitrile; N- (2-chloro-4-methylpiperazino)-N-nitrosoaminoacetonitrile; N-(2-methyl-4-acetylpiperazino)-N- nitroso-aminoacetoriitrile; N-(2-methoxypiperazino)- N-nitroso-aminoacetonitrile; N-(2-acetylpiperazino)- N-nitroso-aminoacetonitrile; N-(2-acetoxypiperazino)- N-nitroso-aminoacetonitrile; N-(2-l3- hydroxyethylpiperazino)-N-nitroso-aminoacetonitrile; N-(2-methoxyethylpiperazino)-N-nitrosoaminoacetonitrile; or N-(3-trifluoromethylpiperazino)- N-nitroso-aminoacetonitrile as the active agent in placev of N-piperazino-N-nitroso-aminoacetonitrile above and are used as above in treating hypertension.

prises orally or parentally administering to a mammal is need of said treatment an anti-hypertensive effective amount of a compound of the formula,

in which R represents hydrogen, alkyl or 15 16 where m is 0, l or 2 and in which R and R together i with N represent (CHHmfl m A 5 where R is hydrogen; lower alkyl; alkanoyl having R N N- Zto 4 carbonatoms; w-hydroxy lower alkyl: alk- ,,,f oxyalkyl having 2 to 4 carbon atoms; lower alkyl having 1 to 4 carbon atoms substituted with l to 4 halogen atoms having an atomic weight between 19 where p is O, l or 2, 10 t0 Or R Represent hydroxy; halo having an atomic weight of about 19 to 36; lower alkyl; lower alkoxy; alkan- I (cn oyl having 2 to 4 carbon atoms; alkanoyloxy having r 2 to 4 carbon atoms; w-hydroxy lower alkyl; alkoxyalkyl having 2 to 4 carbon atoms substituted with l to 4 halogen atoms having an atomic weight between about 19 to 36; or

2. A method according to claim 1 in which the compound is N-(4-B-hydroxyethylpiperazino)-N-nitroso-amethyl-aminoacetonitrile. 1 

1. A METHOD FOR TREATING HYPRTENSION, WHICH COMPRISES ORALLY OR PARENTALLY ADMINISTERING TO A MAMMAL IS NEED OF SAID TREATMENT AN ANTI-HYPERTENSIVE EFFECTIVE AMOUNT OF A COMPOUND OF THE FORMULA,
 2. A method according to claim 1 in which the compound is N-(4- Beta -hydroxyethylpiperazino)-N-nitroso- Alpha -methyl-aminoacetonitrile. 